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Catalog Number | orb1956179 |
---|---|
Category | Proteins |
Description | LIF Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 21.2 kDa and the accession number is P15018. |
Tag | C-His |
Purity | 97.90% |
MW | 21.2 kDa (predicted); 35-42 kDa (reducing conditions) |
UniProt ID | P15018 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | LIF Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 21.2 kDa and the accession number is P15018. |
Expression Region | A DNA sequence encoding the human LIF (P15018) (Met1-Phe202) with a C-terminal polyhistidine tag was expressed. Predicted N terminal: Ser 23 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
98.00% | |
68.94 kDa (predicted). Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result. |
98.00% | |
25.6 kDa (predicted) |
SDS-PAGE: > 99.9%; SEC-HPLC: 92.7% |
98.00% | |
41.9 kDa (predicted); 46 kDa (reducing conditions) |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human LIF R Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gln45-Ser833) of Human LIF R fused with hFc His Tag tag at the C-terminal. |