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Catalog Number | orb1956156 |
---|---|
Category | Proteins |
Description | LIF Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 46.7 kDa and the accession number is P15018. |
Tag | C-hFc |
Purity | 98.00% |
MW | 46.7 kDa (predicted); 63 kDa (reducing conditions) |
UniProt ID | P15018 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | LIF Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 46.7 kDa and the accession number is P15018. |
Expression Region | A DNA sequence encoding the human LIF (P15018)(Met1-Phe202) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Ser 23 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 46 kDa after removal of the signal peptide. The apparent molecular mass of LIF-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 120-140 kDa based on Tris-Bis PAGE result. |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human LIF R Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gln45-Ser833) of Human LIF R fused with hFc His Tag tag at the C-terminal. |