LGR5 antibody (N-term)

• Catalog Number: orb1925279
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: R00662
• Tested applications: WB
• Predicted Reactivity: Mouse
• Reactivity: Hamster
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IHC: 1:500-1:1000, IHC: 1:500-1:1000, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 99998 Da
• Target: This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from human.
• UniProt ID: O75473
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Leucine-rich repeat-containing G-protein coupled r
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-LGR5 antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Observed band size: 100 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of LGR5 Rabbit Brain tissue was performed on the Abcarta FAIP-30 Fully automated IHC platform.Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (Ready-to-use) for 15 min at room temperature.

Immunohistochemical analysis of LGR5 Rabbit Small intestine tissue was performed on the Abcarta FAIP-30 Fully automated IHC platform.Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (Ready-to-use) for 15 min at room temperature.