LGALS3 Antibody (C-term)

• Catalog Number: orb1936454
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB33695
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 26152 Da
• Target: This LGALS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 167-196 amino acids from the C-terminal region of human LGALS3.
• UniProt ID: P17931
• NCBI: NP_002297.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-bin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-LGALS3 Antibody (C-term) at 1:1000 dilution + U118MG whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

LGALS3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the LGALS3 antibody detected the LGALS3 protein (arrow).

LGALS3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of LGALS3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.