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Catalog Number | orb1230641 |
---|---|
Category | Proteins |
Description | Leptin Recombinant Protein |
Form/Appearance | Lyophilized |
Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
Target | Lep |
UniProt ID | P50596 |
Protein Sequence | MVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC |
Source | E. coli |
Biological Activity | Biological activity was determined by an in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5μg leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline - treated controls. |
NCBI | NP_037208.1 |
Storage | The lyophilized Leptin recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted Leptin is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing Leptin recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
Alternative names | OB, obese, Ob, Leptin, Obesity factor Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
> 97 % by SDS-PAGE and HPLC analyses. | |
Approximately 16.0 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids. | |
Escherichia coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
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