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Catalog Number | orb1272416 |
---|---|
Category | Antibodies |
Description | Lep Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Mouse |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant murine Leptin. |
Concentration | batch dependent |
Dilution range | ELISA:To detect Leptin by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Leptin. Sandwich:To detect mLeptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine Leptinas a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin. Western Blot:To detect Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | Lep |
UniProt ID | P41160 |
NCBI | P41160 |
Storage | Leptin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | ob, obese, Ob, Leptin, Obesity factor, Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
To detect mLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Murine Leptin (orb1272415) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin.
To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained 4% PFA, paraffin-embedded sections of Trichuris muris infected murine cecum and large intestine tissue. The primary antibody was incubated at 0.20 ug/ml overnight at 4°C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM citric acid was used.
This antibody stained 4% PFA, paraffin-embedded sections of Trichuris muris infected murine cecum and large intestine tissue. The primary antibody was incubated at 0.20 ug/ml overnight at 4°C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM citric acid was used.
ELISA, ICC, IF, IHC-P | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IHC, WB | |
Canine, Human | |
Goat | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-P, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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