LEF1 antibody

• Catalog Number: orb1151564
• Category: Antibodies
• Description: Rabbit monoclonal antibody to LEF1
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: LEF1/341R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A portion of amino acids 100-200 was used as the immunogen for the LEF1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 2-4ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This LEF1 antibody is available for research use only.
• UniProt ID: Q9UJU2
• Storage: Aliquot the LEF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the LEF1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human lymph node with LEF1 antibody (clone LEF1/341R) at 2 ug/ml in PBS for 30min RT. Strong nuclear staining of non-germinal center observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with LEF1 antibody (clone LEF1/341R) at 2 ug/ml in PBS for 30min RT. Strong nuclear staining of non-germinal center cells is observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.Inset: PBS instead of primary. Secondary antibody negative control.

SDS-PAGE analysis of purified, BSA-free LEF1 antibody (clone LEF1/341R) as confirmation of integrity and purity.