LEF1 Antibody / Lymphoid enhancer-binding factor 1 / TCF1 alpha

• Catalog Number: orb1823433
• Category: Antibodies
• Description: Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells.Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: LEF1/8629R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 1-300) from the human protein was used as the immunogen for the Lymphoid enhancer-binding factor 1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Lymphoid enhancer-binding factor 1 antibody is available for research use only.
• UniProt ID: Q9UJU2
• Storage: Aliquot the Lymphoid enhancer-binding factor 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE analysis of human tonsil tissue with Lymphoid enhancer-binding factor 1 antibody (clone LEF1/8629R). Strong nuclear staining of non-germinal center cells observed. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Lymphoid enhancer-binding factor 1 antibody (clone LEF1/8629R) as confirmation of integrity and purity.