LC3B Antibody, KO Validated

• Catalog Number: orb1274542
• Category: Antibodies
• Description: LC3B Antibody, KO Validated
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide of human LC3B
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 16kDa
• Target: MAP1LC3B
• UniProt ID: Q9GZQ8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: MAP1LC3B Antibody: LC3B, ATG8F, MAP1LC3B-a, MAP1A/
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using LC3B antibody (orb1274543) at 1:1000 dilution. Both C6 cells and NIH/3T3 cells were treated by Chloroquine (50 μM) at 37 ℃ for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (orb1274543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of HeLa cells using LC3B antibody (orb1274543) at dilution of 1:100. Hela cells were treated by Chloroquine (50 μM) for 20 hours (left). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using LC3B antibody (orb1274543) at dilution of 1:100. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours (left). Blue: DAPI for nuclear staining.