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Catalog Number | orb1958487 |
---|---|
Category | Proteins |
Description | Latent TGF beta 1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 42.4 kDa and the accession number is A0A499FJK2. |
Tag | C-His |
Purity | 99.50% |
MW | 42.4 kDa (predicted); 16, 38 and 55 kDa (reducing condition, due to glycosylation); 110 kDa (non-reduced condition, due to glycosylation) |
UniProt ID | P01137 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | Latent TGF beta 1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 42.4 kDa and the accession number is A0A499FJK2. |
Expression Region | A DNA sequence encoding the full length of human TGFβ1 (NP_000651.3) (Met 1-Ser 390) was fused with a C-terminal polyhistidine tag. Predicted N terminal: Leu 30 & Ala 279 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
98.00% | |
108.72 kDa (predicted); (72.33, 40.89 and 13.99) kDa (reducing conditions) |
98.00% | |
42.4 kDa (predicted) |
SDS-PAGE: 74%; SEC-HPLC: 89% | |
130&170 KDa (reducing condition) |
98.00% | |
45.9 kDa (predicted). Due to enzyme lysis and glycosylation, the protein migrates to 43-48 kDa and 50-60 kDa based on Tris-Bis PAGE result. |
98.00% | |
45.9 kDa (predicted). Due to enzyme lysis and glycosylation, the protein migrates to 46-50 kDa and 55-60 kDa based on Tris-Bis PAGE result. |