Laminin antibody

• Catalog Number: orb345361
• Category: Antibodies
• Description: Laminin antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-Laminin Antibody was produced by repeated immunizations with a mixture of synthetic peptides corresponding to regions of adherence on laminin.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:4,000 - 1:8,000, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Laminin has been prepared by immunoaffinity chromatography using immobilized human placental laminin followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian Laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross-reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.
• Conjugation: Unconjugated
• UniProt ID: P07942
• NCBI: AAA59486.1
• Storage: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Laminin antibody, LAMA1 antibody, LAMB
• Note: For research use only
• Application notes: Anti-Laminin (RABBIT) Antibody has been tested by IHC and was assayed by immunoblot and found to be reactive against Laminin at a dilution of 1:5,000 to 1:10,000. Anti-Laminin (Human) (RABBIT) Antibody was also assayed against 1.0 µg of Laminin in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200. Specific conditions should be optimized by researcher.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry of rabbit anti-Laminin antibody. Tissue: human placenta. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Laminin at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: Laminin as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of rabbit anti-Laminin antibody. Tissue: human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Laminin at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: Laminin as precipitated red signal with hematoxylin purple nuclear counterstain.