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| Catalog Number | orb768952 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Laminin alpha-5 |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human LAMA5. AA range:2381-2430 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 400 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-LAMA5 antibody, anti-KIAA0533 antibody, anti- Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.
Western Blot analysis of RAW HepG2 A549 cells using Laminin α-5 Polyclonal Antibody.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using LAMA5 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night.
