Lamin B1/LMNB1 Antibody

• Catalog Number: orb312137
• Category: Antibodies
• Description: Lamin B1/LMNB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Lamin B1 recombinant protein (Position: Q266-C583). Human Lamin B1 shares 95.9% and 95% amino acid (aa) sequence identity with mouse and rat Lamin B1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 66408 MW
• UniProt ID: P20700
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Lamin-B1;LMNB1;LMN2, LMNB;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U937 cells using anti-Lamin B1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Lamin B1 using anti-Lamin B1 antibody.Lane 1:U87 Cell; 2:PC-12 Cell; 3:NIH3T3 Cell.

IF analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in immunocytochemical section of A431 cells.

IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in paraffin-embedded section of Mouse Intestine Tissue.

IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in paraffin-embedded section of Rat Intestine Tissue.

IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in paraffin-embedded section of Human Mammary Cancer Tissue.

IHC analysis of Lamin B1 using anti-Lamin B1 antibody.Lamin B1 was detected in immunocytochemical section of A549 cell.

IHC analysis of Lamin B1 using anti-Lamin B1 antibody.Lamin B1 was detected in immunocytochemical section of HeLa cell.

IHC analysis of Lamin B1 using anti-Lamin B1 antibody.Lamin B1 was detected in immunocytochemical section of SMMC-7721 cell.

IHC analysis of Lamin B1 using anti-Lamin B1 antibody.Lamin B1 was detected in immunocytochemical section of SW480 cell.

IHC analysis of Lamin B1 using anti-Lamin B1 antibody.Lamin B1 was detected in frozen section of human placenta tissue.

IHC analysis of Lamin B1 using anti-Lamin B1 antibody.Lamin B1 was detected in frozen section of mouse small intestine tissue.

IHC analysis of Lamin B1 using anti-Lamin B1 antibody.Lamin B1 was detected in frozen section of rat small intestine tissue.