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LALBA Antibody (Center)
Catalog Number: orb1935445
| Catalog Number | orb1935445 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB34611 |
| Tested applications | IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50, IHC-P: 1:25 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 16225 Da |
| Target | This LALBA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the Central region of human LALBA. |
| UniProt ID | P00709 |
| NCBI | NP_002280.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Alpha-lactalbumin, Lactose synthase B protein, Lys Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
LALBA Antibody (Center) western blot analysis in CEM cell line lysates (35 ug/lane). This demonstrates the LALBA antibody detected the LALBA protein (arrow).
Anti-LALBA Antibody (Center) at 1:2000 dilution + human breast lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 16 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-LALBA Antibody (Center) at 1:2000 dilution + human breast lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 16 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-LALBA Antibody (Center) at 1:2000 dilution + human breast lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 16 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-LALBA Antibody (Center) at 1:2000 dilution + human breast lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 16 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
LALBA Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of LALBA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Staining LALBA in Human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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