LAG3 antibody

• Catalog Number: orb37257
• Category: Antibodies
• Description: LAG3 is a rabbit polyclonal antibody obtained from KLH conjugated synthetic peptide derived from human LAG3. It has various different biological effects on T-cell functioning. It is made of 503 AA. It is expressed on various cells, for example, activated t-cells, plasmacytoid dendritic cells and b cells. It is Involved in lymphocyte activation and binds to HLA class-II antigen. Maintaining of CD8 level is a function carried out by this protein.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB21775
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human LAG3.
• Dilution range: WB~~1:1000, IHC-P~~1:10~50, FC~~1:25
• Form/Appearance: Supplied in PBS with 0.09% (W/V) sodium azide
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 57449 Da
• Target: LAG3
• UniProt ID: P18627
• Expression Region: 103-132 aa
• NCBI: NP_002277.4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-CD223 antibody, anti-CD223 antigen antibody,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of K562 cell line lysates (35ug/lane) using LAG3 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human prostate carcinoma tissue using LAG3 antibody

WB analysis of (lane 1) 293T/17 whole cell lysate (lane 2) mouse heart lysate Lysates using LAG3 antibody

WB analysis of (lane 1) 293T/17 whole cell lysates (lane 2) human spleen lysates using LAG3 antibody

WB analysis of (lane 1) K562 whole cell lysates (lane 2) human liver lysates (lane 3) human ovary lysates using LAG3 antibody

WB analysis of K562 whole cell lysates using LAG3 antibody

WB analysis of (lane 1) human ovary lysate (lane 2) mouse liver lysate Lysates using LAG3 antibody

WB analysis of 293T/17 whole cell lysate Lysates using LAG3 antibody

WB analysis of mouse heart lysate Lysates using LAG3 antibody