LAG3 Antibody

• Catalog Number: orb1806464
• Category: Antibodies
• Description: LAG3 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ICC, IHC, WB
• Reactivity: Mouse
• Immunogen: Between 250 and 300
• Concentration: 1000 µg/ml
• Dilution range: WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: LAG3
• UniProt ID: Q61790
• Storage: Shelf life: 1 year from date of receipt. Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: lymphocyte activation gene 3 protein; Ly66; LAG-3;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse LAG3 by western blot. Samples: Whole cell lysate (50 µg) from BW5147.3, CTLL-2, NIH 3T3, J774A1, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (orb1806464) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse LAG3 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (orb1806464). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse LAG3 by immunocytochemistry. Sample: FFPE section of CTLL-2 cells. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (orb1806464). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse LAG3 by immunocytochemistry. Sample: FFPE section of J774A.1 cells. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (orb1806464). Secondary: HRP-conjugated goat anti-rabbit IgG.