Lag3 Antibody

• Catalog Number: orb1671700
• Category: Antibodies
• Description: Lag3 Antibody
• Clonality: Recombinant
• Clone Number: C9B7W
• Tested applications: Blocking, FC, IP
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising Lewis rats with a murine LAG-3:Ig fusion protein.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Lag3
• UniProt ID: Q61790
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD223, LAG-3, Lymphocyte activation gene 3 protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of mouse splenocytes using anti-Lag3 antibody (C9B7W). Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of C9B7W (orb1671700) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671700- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.

Binding curve of anti-Lag3 antibody C9B7W (orb1671700) to recombinant mouse Lag3 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse Lag3 Fc-Fusion Protein (Pr00162-1.9; Antibody) at a concentration of 2 ug/ml. A 4-fold serial dilution from 5-000 ng/ml was performed using orb1671700. For detection- a 1:4000 dilution of HRP-lnti-ribody was used.