LAG3 Antibody

• Catalog Number: orb1519933
• Category: Antibodies
• Description: LAG3 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ICC, IF, IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 475 and C-terminus
• Concentration: 500 µg/ml
• Dilution range: WB - 1:1000; IP - 12 µl/0.5 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: LAG3
• UniProt ID: P18627
• NCBI: NP_002277.4
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: lymphocyte-activation gene 3; Protein FDC; lymphoc
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human LAG3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-LAG3 recombinant monoclonal antibody (orb1519933) used for IP at 12 µl per reaction.

Detection of human LAG3 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (orb1519933) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).