L1CAM Antibody

• Catalog Number: orb1671662
• Category: Antibodies
• Description: L1CAM Antibody
• Clonality: Recombinant
• Clone Number: L1-14.10
• Tested applications: FC, IF, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: This antibody was raised by immunising mice with recombinant L1-Fc fusion protein, consisting of the ectodomain of human L1.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: L1CAM
• UniProt ID: P32004
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: L1CAM, 14.10, L1, Neural cell adhesion molecule L1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-CD171 antibody L1-14.10. HeLa(A) (0.03 ug/ml)- Kelly(B) cells (0.01 ug/ml)- human brain cerebellum(C) (0.003 ug/ml) and cerebral cortex(D) (0.01 ug/ml) tissue lysate (35 ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of L1-14.10 (orb1671662) before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescence staining of HeLa cells using anti-CD171 (L1-14.10). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric mouse IgG version of L1-14.10 (orb1671662) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom. 1- DAPI- merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (.1) followed by Alexa Fluor® 488 secondary antibody.

Flow cytometry using the Anti-CD171 antibody L1-14.10. Paraformaldehyde fixed HeLa cells were stained with anti-unknown specificity antibody (.1; isotype control - black line) or the mouse IgG1 version of L1-14.10 (orb1671662 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.