L1CAM Antibody (C-term)

• Catalog Number: orb1935060
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB21085
• Tested applications: IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IHC-P: 1~600, WB: 1:1000, WB: 1:8000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 140003 Da
• Target: This L1CAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1154-1182 amino acids from the C-terminal region of human L1CAM.
• UniProt ID: P32004
• NCBI: NP_000416.1, NP_001265045.1, NP_076493.1, NP_001137435.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

L1CAM Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35 ug/lane). This demonstrates the L1CAM antibody detected the L1CAM protein (arrow).

Immunohistochemical analysis of paraffin-embedded Human kidney section using Pink1. Diluted at 1~600 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Anti-L1CAM Antibody (C-term) at 1: 8000 dilution + human fetal brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 140 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.