KRT8 Antibody / Cytokeratin 8

• Catalog Number: orb749367
• Category: Antibodies
• Description: KRT8, also called Cytokeratin 8, is a low molecular weight keratin of approximately 50 kDa. It participates in cellular differentiation and signal transduction, protects against apoptosis, stress and injury, and helps maintain cellular structural integrity. KRT8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: K8.8
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human carcinoma keratin was used as the immunogen for this KRT8 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This KRT8 antibody is available for research use only.
• Entrez: 3856
• Storage: Store the KRT8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Differences in protocols and secondaries may require the KRT8 antibody to be titered for optimal performance.1. FFPE staining requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of human colon carcinoma stained with KRT8 antibody (K8.8).

Immunofluorescent staining of permeabilized human HCT-116 cells with KRT8 antibody (clone K8.8, green) and Reddot nuclear stain (red).

Flow cytometry testing of permeabilized human HeLa cells with KRT8 antibody (clone K8.8); Red=isotype control, Blue=KRT8 antibody.

Western blot testing of HCT-116 cell lysate using KRT8 antibody (clone K8.8) at 1 ug/ml. Observed molecualr weight: 46~50kDa.