KRT8 Antibody / Cytokeratin 8

• Catalog Number: orb606391
• Category: Antibodies
• Description: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibody that recognizes only cytokeratin 8 and 18. Cytokeratin 8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: KRT8/2174R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A cytoskeletal preparation from human HeLa cells was used as the immunogen for this recombinant Cytokeratin 8 antibody.
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Cytokeratin 8 antibody is available for research use only.
• UniProt ID: P05787
• Storage: Store the recombinant Cytokeratin 8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Cytokeratin 8 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human HCT-116 cell lysate with recombinant Cytokeratin 8 antibody (clone KRT8/2174R).

SDS-PAGE analysis of purified, BSA-free recombinant Cytokeratin 8 antibody (clone KRT8/2174R) as confirmation of integrity and purity.

IHC testing of FFPE human small intestine tissue with recombinant Cytokeratin 8 antibody (clone KRT8/2174R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.