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Catalog Number | orb1252385 |
---|---|
Category | Antibodies |
Description | KRT76 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | KRTH/1076 |
Tested applications | ELISA, FC, IF, IHC-P, WB |
Reactivity | Human, Rat |
Isotype | IgG1, kappa |
Immunogen | Recombinant human KRT76 protein was used as the immunogen for the Basic Cytokeratin antibody. |
Concentration | 0.2 mg/mL |
Dilution range | ELISA (For coating order Ab without BSA)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Basic Cytokeratin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | KRT76 |
UniProt ID | Q01546 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | KRT2B, KRT2P, Keratin, type II cytoskeletal 2 oral Read more... |
Note | For research use only |
Application notes | ELISA (For coating order Ab without BSA)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Basic Cytokeratin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Formalin paraffin human skin stained with Basic Cytokeratin antibody (KRTH/1076).
Formalin paraffin rat stomach stained with Basic Cytokeratin antibody (KRTH/1076).
Formalin paraffin rat oviduct with Basic Cytokeratin antibody (KRTH/1076).
SDS-PAGE Analysis of Purified, BSA-Free Basic Cytokeratin Antibody (clone KRTH/1076). Confirmation of Integrity and Purity of the Antibody.
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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