KRT76 Antibody

• Catalog Number: orb1252057
• Category: Antibodies
• Description: KRT76 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BCCK1-1
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Rat
• Isotype: IgG1, kappa
• Immunogen: An amino acid sequence common to basic keratins was used as the immunogen for the Basic Cytokeratin antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 1-2 ug/ml (3)Immunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the Basic Cytokeratin antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. This Basic Cytokeratin antibody detects the following keratins/CKs: CK1 (67kDa), CK3 (64kDa), CK4 (59kDa), CK5 (58 kDa), CK6 (56kDa), CK8 (52 kDa).
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: KRT76
• UniProt ID: Q01546
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: KRT2B, KRT2P, Keratin, type II cytoskeletal 2 oral
• Note: For research use only
• Application notes: Western blot: 1-2 ug/ml (3)Immunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the Basic Cytokeratin antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. This Basic Cytokeratin antibody detects the following keratins/CKs: CK1 (67kDa), CK3 (64kDa), CK4 (59kDa), CK5 (58 kDa), CK6 (56kDa), CK8 (52 kDa).
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human breast carcinoma with Basic Cytokeratin antibody.

IHC testing of FFPE rat stomach with Basic Cytokeratin antibody.