KRT7 Antibody

• Catalog Number: orb1274234
• Category: Antibodies
• Description: KRT7 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: KRT7/1499R
• Tested applications: FC, IF, IHC-P
• Reactivity: Human
• Isotype: IgG, kappa
• Immunogen: Full-length human CK7 protein was used as the immunogen for the recombinant Cytokeratin 7 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1ug/10e6 cellsImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant Cytokeratin 7 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: KRT7
• UniProt ID: P08729
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Keratin, type II cytoskeletal 7, Cytokeratin-7, CK
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1ug/10e6 cellsImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant Cytokeratin 7 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human oviduct with recombinant Cytokeratin 7 antibody (clone KRT7/1499R). Required HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

Western blot testing of human HeLa cell lysate with recombinant Cytokeratin 7 antibody (clone KRT7/1499R).

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Cytokeratin 7 antibody (clone KRT7/1499R); Red = isotype control, Blue = recombinant Cytokeratin 7 antibody.

SDS-PAGE analysis of purified, BSA-free recombinant Cytokeratin 7 antibody (clone KRT7/1499R) as confirmation of integrity and purity.