KRT7 Antibody

• Catalog Number: orb1254673
• Category: Antibodies
• Description: KRT7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 230-469 of human KRT7 (NP_005547.3).
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 51kDa
• Target: KRT7
• UniProt ID: P08729
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: Keratin, type II cytoskeletal 7, Cytokeratin-7, CK
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of HeLa cells, using KRT7 antibody (orb1254673). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded human placenta using KRT7 antibody (orb1254673) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of human lung cancer cells using KRT7 antibody (orb1254673) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat bronchus cells using KRT7 antibody (orb1254673) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse bronchus cells using KRT7 antibody (orb1254673) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of human lung cancer cells using KRT7 antibody (orb1254673) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat bronchus cells using KRT7 antibody (orb1254673) at dilution of 1:100. Blue: DAPI for nuclear staining.