KRT7 Antibody / Cytokeratin 7

• Catalog Number: orb749625
• Category: Antibodies
• Description: It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KRT7/760
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human KRT7 protein was used as the immunogen for the Cytokeratin 7 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Cytokeratin 7 antibody is available for research use only.
• UniProt ID: P08729
• Storage: Store the Cytokeratin 7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with Cytokeratin 7 antibody (clone KRT7/760).

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin 7 antibody (clone KRT7/760).

IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Cytokeratin 7 antibody (clone KRT7/760).

Western blot testing of human HeLa cell lysate with Cytokeratin 7 antibody (clone KRT7/760). Predicted molecular weight ~51 kDa.

Flow cytometry testing of PFA-fixed human HeLa cells with Cytokeratin 7 antibody (clone KRT7/760); Red=isotype control, Blue=recombinant Cytokeratin 7 antibody.

Immunofluorescent staining of methanol-fixed human HeLa cells with Cytokeratin 7 antibody (clone KRT7/760, green) and Reddot nuclear stain (red).