KRT7 Antibody / Cytokeratin 7

• Catalog Number: orb749621
• Category: Antibodies
• Description: CK7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Expressed in cultured epidermal, bronchial and mesothelial cells. [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: OV-TL12/30
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: OTN 11 cells (ovarian carcinoma cell line) were used as the immunogen for the Cytokeratin 7 antibody.
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Cytokeratin 7 antibody is available for research use only.
• UniProt ID: P08729
• Storage: Store the Cytokeratin 7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The stated application concentrations are suggested starting amounts. Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. View the recombinant version of this Cytokeratin 7 antibody.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of methanol-fixed human HeLa cells with Cytokeratin 7 antibody.

IHC: Formalin-fixed, paraffin-embedded ovarian carcinoma stained with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC: Formalin-fixed, paraffin-embedded human lung SCC stained with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Flow cytometry testing of PFA-fixed human HeLa cells with Cytokeratin 7 antibody (clone OV-TL12/30); Red = isotype control, Blue = recombinant Cytokeratin 7 antibody.

Western blot testing of human HeLa cell lysate with Cytokeratin 7 antibody (clone OV-TL12/30).

SDS-PAGE analysis of purified, BSA-free Cytokeratin 7 antibody (clone OV-TL12/30) as confirmation of integrity and purity.