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Catalog Number | orb1825736 |
---|---|
Category | Antibodies |
Description | It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract.This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-). |
Species/Host | Mouse |
Clonality | Recombinant |
Clone Number | rKRT7/8763 |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Mouse IgG2b, kappa |
Immunogen | Recombinant full-length human KRT7 protein was used as the immunogen for the recombinant KRT7 antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT |
Conjugation | Unconjugated |
Formula | 1 mg/ml in 1X PBS; BSA free, sodium azide free |
Hazard Information | This recombinant KRT7 antibody is available for research use only. |
UniProt ID | P08729 |
Storage | Aliquot the recombinant KRT7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human prostate carcinoma tissue with recombinant KRT7 antibody (clone rKRT7/8763). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant KRT7 antibody (clone rKRT7/8763) as confirmation of integrity and purity.
FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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