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Catalog Number | orb1252495 |
---|---|
Category | Antibodies |
Description | KRT19 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | KRT19/799 |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG2a, kappa |
Immunogen | Recombinant human KRT19 protein was used as the immunogen for the Cytokeratin 19 antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Cytokeratin 19 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | KRT19 |
UniProt ID | P08727 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Keratin, type I cytoskeletal 19, Cytokeratin-19, C Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Cytokeratin 19 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 19 antibody (KRT19/799).
Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Cytokeratin 19 antibody (KRT19/799).
Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Cytokeratin 19 antibody (KRT19/799).
Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin 19 antibody (KRT19/799).
Formalin-fixed, paraffin-embedded human pancreas stained with Cytokeratin 19 antibody (KRT19/799).
SDS-PAGE Analysis of Purified, BSA-Free Cytokeratin 19 Antibody (clone KRT19/799). Confirmation of Integrity and Purity of the Antibody.
FC, IF | |
Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
FITC |
IF, IHC-P, WB | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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