KRT19 Antibody / Cytokeratin 19

• Catalog Number: orb749641
• Category: Antibodies
• Description: Recognizes a protein of 40kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KRT19/799
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, lambda
• Immunogen: Recombinant human KRT19 protein was used as the immunogen for the Cytokeratin 19 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Cytokeratin 19 antibody is available for research use only.
• UniProt ID: P08727
• Storage: Store the Cytokeratin 19 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Cytokeratin 19 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 19 antibody (KRT19/799).

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Cytokeratin 19 antibody (KRT19/799).

IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Cytokeratin 19 antibody (KRT19/799).

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin 19 antibody (KRT19/799).

IHC: Formalin-fixed, paraffin-embedded human pancreas stained with Cytokeratin 19 antibody (KRT19/799).

Immunofluorescent staining of permeabilized human MCF7 cells with Cytokeratin 19 antibody (clone KRT19/799, green) and Reddot nuclear stain (red).

Flow cytometry testing of methanol-fixed human MCF7 cells with Cytokeratin 19 antibody (clone KRT19/799); Red=isotype control, Blue=Cytokeratin 19 antibody.

Western blot testing of human HepG2 antibody with Cytokeratin 19 antibody (clone KRT19/799). Predicted molecular weight ~43 kDa.

SDS-PAGE analysis of purified, BSA-free Cytokeratin 19 antibody (clone KRT19/799) as confirmation of integrity and purity.