KRT19 Antibody / Cytokeratin 19

• Catalog Number: orb385888
• Category: Antibodies
• Description: Cytokeratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Cytokeratin 19 is a type I keratin. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically found in the periderm, the transiently superficial layer that envelops the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [Wiki]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CTKN19-1
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant human KRT19 protein was used as the immunogen for the Keratin 19 antibody.
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Keratin 19 antibody is available for research use only.
• UniProt ID: P08727
• Storage: Store the Keratin 19 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the Keratin 19 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of permeabilized human MCF7 cells with Cytokeratin 19 antibody (clone CTKN19-1, green) and Reddot nuclear stain (red).

Flow cytometry testing of methanol-fixed human MCF7 cells with Cytokeratin 19 antibody (clone CTKN19-1); Red=isotype control, Blue= Cytokeratin 19 antibody.

Western blot testing of human HepG2 antibody with Cytokeratin 19 antibody (clone CTKN19-1). Predicted molecular weight ~43 kDa.

IHC testing of FFPE human pancreas and Keratin 19 antibody (clone CTKN19-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

IHC testing of FFPE human ovarian carcinoma and Keratin 19 antibody (clone CTKN19-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.