KRT15 Antibody / Cytokeratin 15

• Catalog Number: orb639964
• Category: Antibodies
• Description: Cytokeratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CTKN15-1
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2c, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the Cytokeratin 15 antibody.
• Dilution range: Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Cytokeratin 15 antibody is available for research use only.
• UniProt ID: P19012
• Storage: Store the Cytokeratin 15 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human thymus tissue and HCT-116 cell lysate with CK15 antibody (clone CTKN15-1). Expected molecular weight ~50 kDa.

Immunofluorescent staining of MeOH-fixed human MCF7 cells with Cytokeratin 15 antibody (clone CTKN15-1, green) and Reddot nuclear stain (red).

IHC staining of FFPE human prostate carcinoma with Cytokeratin 15 antibody (clone CTKN15-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.