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Catalog Number | orb606440 |
---|---|
Category | Antibodies |
Description | Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | KRT15/2103R |
Tested applications | FACS, IHC-P |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | Recombinant human protein was used as the immunogen for the recombinant Cytokeratin 15 antibody. |
Dilution range | Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This recombinant Cytokeratin 15 antibody is available for research use only. |
UniProt ID | P19012 |
Storage | Store the recombinant Cytokeratin 15 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the recombinant Cytokeratin 15 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human skin with recombinant Cytokeratin 15 antibody (clone KRT15/2103R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human prostate tissue with recombinant Cytokeratin 15 antibody (clone KRT15/2103R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant Cytokeratin 15 antibody (clone KRT15/2103R) as confirmation of integrity and purity.
FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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