KRAS antibody

• Catalog Number: orb527166
• Category: Antibodies
• Description: Rabbit polyclonal antibody to KRAS
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB32708
• Tested applications: FC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: WB: 1:2000, IF: 1:10-50, FC: 1:10-50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 21656
• Target: KRAS
• UniProt ID: P01116
• NCBI: NP_203524.1, NP_004976.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, G
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Confocal immunofluorescent analysis of KRAS Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

All lanes:Anti-KRAS Antibody (C-term) at 1:500 dilution.Lane 1:Hela;Lane 2:293T/17;Lane 3:C2C12;Lane 4:C6;Lane 5:HT-29;Lane 6:PC-12.

Western blot:Anti-KRAS Antibody (C-term) at 1:2000 dilution + HT-29.Secondary antibody was Goat Anti-Rabbit IgG, (H+L).

Western blot:All lanes:Anti-KRAS Antibody (C-term) at 1:2000 dilution.Lane 1:293T/17;Lane 2:C2C12;Lane 3:Hela;Lane 4:HT-29;Lane 5:K562;Lane 5:PC-12;Lane 5:Ramos.

Flow cytometric analysis of Hela cells (right histogram) using KRAS Antibody (C-term), compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.