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Catalog Number | orb1535363 |
---|---|
Category | Antibodies |
Description | KRAS Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC, IHC-P, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant human GTPase KRas protein. |
Concentration | 4 mg/ml |
Dilution range | ELISA, IF, IHC, IHC-P (10 µg/ml), WB |
Conjugation | Unconjugated |
Target | KRAS |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.. PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. |
Alternative names | KRAS, C-K-RAS, C-Ki-ras, C-Kirsten-ras protein, GT Read more... |
Note | For research use only |
Application notes | Further information: Recommended conditions for Immunohistochemistry: For FFPE tissues, perform steam-based antigen retrieval using Tris/EDTA buffer at pH 9.0. |
Expiration Date | 12 months from date of receipt. |
Anti-KRAS antibody IHC staining of human small intestine.
Immunofluorescence staining of Hela cells with Rabbit anti-human GTPase KRas polyclonal Antibody at 1:133, counter-stained with DAPI.
Western blot All lanes: KRAS antibody at 2 µg/ml Lane 1: Mouse brain tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa
FC, IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |