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Catalog Number | orb1938024 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB18408 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 71957 Da |
Target | This KNG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-166 amino acids from the N-terminal region of human KNG1. |
UniProt ID | P01042 |
NCBI | NP_001159923.1, NP_000884.1, NP_001095886.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Kininogen-1, Alpha-2-thiol proteinase inhibitor, F Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-KNG1 Antibody (N-term) at 1:1000 dilution + Ramos whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 72 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
KNG1 Antibody (N-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
KNG1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of KNG1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of KNG1 (arrow) using rabbit polyclonal KNG1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KNG1 gene.