KLK7 antibody

• Catalog Number: orb2276259
• Category: Antibodies
• Description: KLK7 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-707M
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG2b, kappa
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P49862
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: Kallikrein related peptidase 7 (HGNC Symbol), PRSS
• Note: For research use only
• Application notes: Positive Control: Skin: A strong KLK7 staining should be seen in the stratum granulosum and possibly also in the keratinizing cell layers. Negative Control: Colon: KLK7 immunostaining should be absent in all cells of the colon mucosa Cellular Localization: Intracelullar Secreted Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

A strong KLK7 staining in the stratum granulosum and the keratinizing superficial layer of the skin.

KLK7 staining is absent in all cells of the colon mucosa.

Squamous cell carcinoma showing strong KLK7 staining in areas of keratinization.