KLF6 antibody

• Catalog Number: orb667101
• Category: Antibodies
• Description: Rabbit polyclonal antibody to KLF6
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse
• Immunogen: Recombinant fusion protein of human KLF6. The exact sequence is proprietary.
• Dilution range: WB: 1-500-2000, IF: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: KLF6
• Entrez: 1316
• UniProt ID: Q99612, O08584
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-BCD1 antibody, anti-COPEB antibody, anti-CPBP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of KLF6 expression in PC3 (A) whole cell lysates. (Predicted band size: 26; 30; 31 kD; Observed band size: 45 kD)

Immunofluorescent analysis of KLF6 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.