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Catalog Number | orb1272656 |
---|---|
Category | Antibodies |
Description | Kitlg Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, NeA, WB |
Reactivity | Rat |
Immunogen | Produced from sera of rabbits immunized with highly pure recombinant Rat IP-10. Rat IP-10 specific antibody was purified by affinity chromatography employing an immobilized Rat IP-10 matrix. |
Concentration | batch dependent |
Dilution range | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rat SCF (50.0 ng/mL), a concentration of 1.2-1.8 μg/mL of this antibody is required. ELISA:Indirect:To detect rat SCF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF. SandwichTo detect rat SCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat SCF (38-258) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF. Western Blot:To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | Kitlg |
UniProt ID | P21581 |
NCBI | P21581 |
Storage | The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | Mgf, SCF, Kitl, Mgf, Kit ligand, Hematopoietic gro Read more... |
Note | For research use only |
Application notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rat SCF (50.0 ng/mL), a concentration of 1.2-1.8 μg/mL of this antibody is required. ELISA:Indirect:To detect rat SCF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF. SandwichTo detect rat SCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat SCF (38-258) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF. Western Blot:To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
To detect rat SCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat SCF (orb1272649) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF.
To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA, IF, IHC-P, WB | |
Bovine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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