Kitlg Antibody (Biotin)

• Catalog Number: orb1272649
• Category: Antibodies
• Description: Kitlg Antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Rat
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Rat SCF. Rat SCF specific antibody was purified by affinity chromatography and then biotinylated.
• Concentration: batch dependent
• Dilution range: To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Biotin
• Target: Kitlg
• UniProt ID: P21581
• NCBI: P21581
• Storage: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: Mgf, SCF, Kitl, Mgf, Kit ligand, Hematopoietic gro
• Note: For research use only
• Application notes: To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect rat SCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat SCF (orb1272656) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant rat SCF.