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Catalog Number | orb1248358 |
---|---|
Category | Antibodies |
Description | KIT/CD117 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, WB |
Predicted Reactivity | Bovine, Canine, Porcine |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: KRDSFICSKQEDH |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 110kDa according to NP_000213.1). An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HEK293 and HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Approx 110kDa |
Target | KIT / CD117 |
UniProt ID | P10721 |
NCBI | NP_000213.1, NP_001087241.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | KIT, v-kit Hardy-Zuckerman 4 feline sarcoma viral Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 110kDa according to NP_000213.1). An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HEK293 and HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1248358 (1 ug/ml) staining of Human Hippocampus lysate (A) + peptide (B) (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248358 Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic/membrane staining.
orb1248358 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic/membrane staining.
orb1248358 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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