KIF5B antibody

• Catalog Number: orb750522
• Category: Antibodies
• Description: KIF5B antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: Antiserum
• Immunogen: Anti-Kinesin-1 was prepared from whole rabbit serum produced by repeated immunizations with a truncated kinesin-1 construct expressed in E. coli corresponding to human kinsesin-1 protein.
• Concentration: 77 mg/mL
• Dilution range: ELISA: 1:10000, IHC: User Optimized, WB: 1:100-1:500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Kinesin-1 is directed against the human kinesin-1 protein. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest reactivity with human. Cross-reactivity with kinesin 1 from other sources have not been determined.
• Conjugation: Unconjugated
• UniProt ID: P33176
• NCBI: NP_004512.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Kinesin 1 Antibody, Conventional kines
• Note: For research use only
• Application notes: Kinesin-1 antibody has been tested for use in SDS, western blot, and immunohistochemistry. For western blots expect a band of approximately 72 kDa in size corresponding to truncated kinesin-1 protein. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry of Rabbit anti-Kinesin-1. Tissue: Human Brain at 40X at pH 6.

SDS-PAGE of Truncated Kinesin 1 Protein. Lane 1: Truncated Kinesin-1 Protein. Lane 2: none. Load: 5 ug per lane. Block: coomassie blue for 20 min, destain overnight at RT. Predicted/Observed size: 72 kDa, 72 kDa for kinesin-1. Other band(s): degradation.

Western Blot of Rabbit anti-Kinesin 1 Antibody. Lane 1: 500 ng of truncated kinesin 1 protein. Lane 2: 20 ug of Mouse Brain lysate. Lane 3: 10 ug of Mouse Brain lysate. Primary antibody: kinesin1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:40000 for 1 hr at RT. Block: 5% BLOTTO 1 hr at RT. Predicted/Observed size: 72 kDa, ~70 kDa for kinesin-1. Other band(s): degradation.