Ki67 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb2563513
• Category: Antibodies
• Description: Ki67 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ICC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Ki67
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:500-1000, IHC-F=1:500-1000, ICC/IF=1:200-500, IF=1:300-1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 358 kDa
• Target: MKI67
• UniProt ID: P46013
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Antigen identified by monoclonal antibody Ki 67; A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

4% Paraformaldehyde-fixed Hela (H) cell, Triton X-100 at r.t. for 20 min, Antibody incubation with (Ki-67) monoclonal Antibody, unconjugated (orb2563513) 1:200, 90 min at 37°C, followed by conjugated Goat Anti-Rabbit IgG antibody (green, orb868805) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

(Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Herat, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67 Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

(Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Herat, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67 Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. PBS instead of the primary antibody was used as the blank control.

Paraformaldehyde-fixed, paraffin embedded Rat Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Skin, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.