Ki-67 antibody

• Catalog Number: orb1151577
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Ki-67
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: MKI67/4945R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A portion of amino acids 2293-2478 from the human protein was used as the immunogen for the recombinant Ki-67 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Ki-67 antibody is available for research use only.
• UniProt ID: P46013
• Storage: Aliquot the recombinant Ki-67 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Ki-67 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil tissue with recombinant Ki-67 antibody (clone MKI67/4945R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant Ki-67 antibody (clone MKI67/4945R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymph node tissue with recombinant Ki-67 antibody (clone MKI67/4945R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human Raji and Ramos cell lysates using recombinant Ki-67 antibody (clone MKI67/4945R).

SDS-PAGE analysis of purified, BSA-free recombinant Ki-67 antibody (clone MKI67/4945R) as confirmation of integrity and purity.