KDM6A antibody

• Catalog Number: orb412686
• Category: Antibodies
• Description: Rabbit polyclonal antibody to KDM6A
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human KDM6A
• Dilution range: WB: 1:500-2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: KDM6A
• Entrez: 7403, 22289
• UniProt ID: O15550, O70546
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-UTX antibody, anti-Lysine-specific demethylas
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of KDM6A expression in SHSY5Y (A), NIH3T3 (B), mouse spleen (C), rat liver (D) whole cell lysates. (Predicted band size: 154; 157 kD; Observed band size: 154 kD)

Immunohistochemical analysis of KDM6A staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.