JAK2 (pY221) antibody

• Catalog Number: orb665512
• Category: Antibodies
• Description: Rabbit polyclonal antibody to JAK2 (Phospho-Y221)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y221 of human JAK2 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1000, IHC-P: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: JAK2
• Entrez: 3717, 24514, 16452
• UniProt ID: Q62689, Q62120, O60674
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-Tyrosine-protein kinase JAK2 antibody, anti-J
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of JAK2 (Phospho-Y221) expression in HepG2 (A), LO2 (B) whole cell lysates. (Predicted band size: 130 kD; Observed band size: 125 kD)

Immunohistochemical analysis of JAK2 (Phospho-Y221) staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Direct ELISA antibody dose-response curve using Anti-JAK2 (Phospho-Y221) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.