JAK2 (phospho-Y570) antibody

• Catalog Number: orb214136
• Category: Antibodies
• Description: Rabbit polyclonal antibody to JAK2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rabbit, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y570 of human JAK2 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: JAK2
• Entrez: 24514, 16452, 3717
• UniProt ID: Q62689, O60674, Q62120
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Tyrosine-protein kinase JAK2 antibody, anti J
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of JAK2 (Phospho-Y570) expression in HeLa (A), TF1 (B), rat kidney (C) whole cell lysates. (Predicted band size: 130 kD; Observed band size: 125 kD)

Immunohistochemical analysis of JAK2 (Phospho-Y570) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.