JAG1 Antibody

• Catalog Number: orb1248305
• Category: Antibodies
• Description: JAG1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-TNKQDNRDLESAQS
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: JAG1
• UniProt ID: P78504
• NCBI: NP_000205.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: JAG1, jagged 1, AGS, AHD, AWS, CD339, HJ1, JAGL1,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Immunohistochemistry:In paraffin embedded Human Kidney shows membrane staining in the epithelial cells of convoluted tubules. Recommended concentration, 2-4ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248305 (2 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1248305 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.