ITGAX Antibody (C-term)

• Catalog Number: orb1929673
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB21771
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 127829 Da
• Target: This ITGAX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1119-1145 amino acids from the C-terminal region of human ITGAX.
• UniProt ID: P20702
• NCBI: NP_000878.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Integrin alpha-X, CD11 antigen-like family member
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of SK-Br-3 cells using ITGAX Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence analysis of ITGAX Antibody (C-term) with paraffin-embedded human hepatocarcinoma.0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with ITGAX Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of ITGAX Antibody (C-term) in MDA-MB231 cell line lysates (35 ug/lane). ITGAX (arrow) was detected using the purified Pab.